Des bibliothèques inclusives

De lintégration à linclusion, le pas franchi est celui qui traduit dans les pratiques avec les publics la révolution copernicienne qui a bouleversé la bibliothéconomie en plaçant les usagers « au centre ». Sa mission émancipatrice passe désormais par la co-construction des services

Didactique de l’écriture en situation de raccrochage scolaire : une entrée par les compétences. Un début d’étude collaborative au micro lycée de Paris

International audienceDidactique de l'écriture en situation de raccrochage : une entrée par les compétences ? Un début d'étude collaborative au micro-lycée de Paris Régine Delamotte, Marie-Claude Penloup et Anne-Marie Petitjean, université de Rouen La question du passage à l'écriture s'avère peu traitée dans les travaux actuels sur le décrochage scolaire alors même que les enseignants des micro-lycées d'Évreux et de Paris ont une demande explicite concernant les difficultés que rencontrent leurs élèves pour passer à l'écriture acadé-mique, en même temps d'ailleurs qu'ils soulignent leurs compétences en écriture créative. Pour répondre à cette demande, l'étude diagnostique que nous présentons s'appuie sur des données diverses : observations, enquêtes auprès des acteurs, textes produits lors d'une séance d'écriture créative. Cet ensemble est construit à partir de positions théoriques sociolinguistiques et (so-cio)didactiques qui seront rappelées. Il nous amène à mettre en évidence l'existence effective de compétences en écriture créative chez les élèves du micro-lycée et à en évaluer les enjeux pour la didactique

Similarities and differences in the biochemical and enzymological properties of the four isomaltases from Saccharomyces cerevisiae

AbstractThe yeast Saccharomyces cerevisiae IMA multigene family encodes four isomaltases sharing high sequence identity from 65% to 99%. Here, we explore their functional diversity, with exhaustive in-vitro characterization of their enzymological and biochemical properties. The four isoenzymes exhibited a preference for the α-(1,6) disaccharides isomaltose and palatinose, with Michaëlis–Menten kinetics and inhibition at high substrates concentration. They were also able to hydrolyze trisaccharides bearing an α-(1,6) linkage, but also α-(1,2), α-(1,3) and α-(1,5) disaccharides including sucrose, highlighting their substrate ambiguity. While Ima1p and Ima2p presented almost identical characteristics, our results nevertheless showed many singularities within this protein family. In particular, Ima3p presented lower activities and thermostability than Ima2p despite only three different amino acids between the sequences of these two isoforms. The Ima3p_R279Q variant recovered activity levels of Ima2p, while the Leu-to-Pro substitution at position 240 significantly increased the stability of Ima3p and supported the role of prolines in thermostability. The most distant protein, Ima5p, presented the lowest optimal temperature and was also extremely sensitive to temperature. Isomaltose hydrolysis by Ima5p challenged previous conclusions about the requirement of specific amino acids for determining the specificity for α-(1,6) substrates. We finally found a mixed inhibition by maltose for Ima5p while, contrary to a previous work, Ima1p inhibition by maltose was competitive at very low isomaltose concentrations and uncompetitive as the substrate concentration increased. Altogether, this work illustrates that a gene family encoding proteins with strong sequence similarities can lead to enzyme with notable differences in biochemical and enzymological properties

Sources of dissolved organic matter during storm and inter-storm conditions in a lowland headwater catchment: constraints from high-frequency molecular data

International audienceThe transfer of dissolved organic matter (DOM) at soil–river interfaces controls the biogeochemistry of mi-cropollutants and the equilibrium between continental and oceanic C reservoirs. Understanding the mechanisms controlling this transfer is fundamental to ecology and geochem-istry. DOM delivery to streams during storms is assumed to come from the flushing of preexisting soil DOM reservoirs mobilized by the modification of water flow paths. We tested this hypothesis by investigating the evolution of the composition of stream DOM during inter-storm conditions and five storm events monitored with high-frequency sampling. The composition of DOM was analyzed using thermally assisted hydrolysis and methylation (THM) with tetramethylammo-nium hydroxide (TMAH) coupled to a gas chromatograph and mass spectrometer. In inter-storm conditions, stream DOM is derived from the flushing of soil DOM, while during storm events, the modification of the distribution of chemical biomarkers allows the identification of three additional mechanisms. The first one corresponds to the destabilization of microbial biofilms due to the increase in water velocity, resulting in the fleeting export of a microbial pool. The second mechanism corresponds to the erosion of soils and river banks, leading to a partition of organic matter between particulate and dissolved phases. The third mechanism is linked to the increase in water velocity in soils that could induce the erosion of macropore walls, leading to an in-soil partition between soil microparticles and dissolved phase. The contribution of this in-soil erosive process would be linked to the magnitude of the hydraulic gradient following the rise of the water table and could persist after the recession, which could explain why the return to inter-storm composition of DOM does not follow the same temporal scheme as the discharge. These results are the most important factors in understanding the transfer of nutrients and micropollutants at the soil–river interfaces during the hot moments that are storm events

Hydrodynamical Simulations of the Lyman Alpha Forest: Model Comparisons

We investigate the properties of the Lyman alpha forest as predicted by numerical simulations for a range of currently viable cosmological models. This is done in order to understand the dependencies of the forest on cosmological parameters. Focusing on the redshift range from two to four, we show that: (1) most of the evolution in the distributions of optical depth, flux and column density can be understood by simple scaling relations, (2) the shape of optical depth distribution is a sensitive probe of the amplitude of density fluctuations on scales of a few hundred kpc, (3) the mean of the b distribution (a measure of the width of the absorption lines) is also very sensitive to fluctuations on these scales, and decreases as they increase. We perform a preliminary comparison to observations, where available. A number of other properties are also examined, including the evolution in the number of lines, the two-point flux distribution and the HeII opacity.Comment: 37 pages, 21 figures, submitted to Ap

Inflammatory control in AIDS-resistant non human primates

International audienceAfrican non human primates are natural hosts of SIV. The infection is non-pathogenic despite plasma viral load levels similar to those in HIV-1 infected humans and SIVmac-infected macaques (MAC) progressing towards AIDS. The most striking difference between non-pathogenic SIV and pathogenic HIV-1/SIVmac infections is the lack of chronic T cell activation in natural hosts. In HIV and SIVmac infections, chronic T cell activation is known to drive CD4+T cell depletion. Intense research efforts are worldwide put on the search of the mechanisms that can control chronic T cell activation in HIV/SIV infections. Innate immune responses play a determinant role in the regulation of T cell activation profiles. Type I interferons (IFN-I) are part of the first-wave response of the innate immune system in viral infections. We compared the IFN-I responses between pathogenic (MAC) and non-pathogenic SIV infections (African Green monkey, AGM) at the level of blood and lymph nodes (LN) during the early and chronic stage of infection. During the acute SIVagm infection, we detected high amounts of IFN-α in the plasma of AGMs, although the mean levels at the peak were three times lower than in MAC. The microarray data revealed a rapid and strong up-regulation of type I Interferon-Stimulated Genes (ISG) in AGMs during acute SIVagm infection. ISGs denote the in vivo activity of IFN-I. Using a functional assay, we demonstrated that low IFN-α concentrations (50 times lower than the IFN-α levels in plasma at the peak) were sufficient to induce strong ISG responses in AGM and MAC cells. Surprisingly, our direct comparison of blood and LNs showed that ISG induction was broader in blood of AGMs than in MAC, while in LN, it was the contrary. Thus, in AGMs, less ISG were induced in LNs as compared to MAC already during the acute phase of infection. Moreover, our tight kinetic analysis showed that this ISG expression was efficiently controlled after day 28 post-infection in AGMs, while in MAC the ISGs expression remained uncontrolled. Finally, we identified genes that were differentially expressed between the two species and which might be involved in the discriminating responses. Altogether, this shows that AGMs are capable to mount a well coordinated and efficient regulative response to innate immune activation